Templated assembly of albumin-based nanoparticles for simultaneous gene silencing and magnetic resonance imaging

D Mertz; C Affolter-Zbaraszczuk; J Barthès; J Cui; F Caruso; TF Baumert; JC Voegel; J Ogier; F Meyer

Journal article

Templated assembly of albumin-based nanoparticles for simultaneous gene silencing and magnetic resonance imaging

D Mertz, C Affolter-Zbaraszczuk, J Barthès, J Cui, F Caruso, TF Baumert, JC Voegel, J Ogier, F Meyer

Nanoscale | Published : 2014

DOI: 10.1039/c4nr02623c

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Abstract

In this article, we address the design of innovative human serum albumin (HSA)-based nanoparticles loaded with silencing RNA and grafted with gadolinium complexes having average sizes ranging from ca. 50 to 150 nm according to the siRNA/HSA composition. The non-covalent siRNA/HSA assembly is formed on isobutyramide-modified mesoporous silica and the self-supported HSA-based nanoparticles are obtained following the silica template dissolution. These original protein particles provide simultaneous magnetic resonance imaging contrast enhancement and cellular in vitro gene silencing.

University of Melbourne Researchers

Frank Caruso Author

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Grants

Awarded by Lab Ex HEPSYS, France

Awarded by Australian Research Council

Funding Acknowledgements

We gratefully thank Franck Blindauer for MRI imaging, Anne Boos and Pascale Ronot for ICP-AES analysis. This work was financially supported by IHU Strasbourg and "Association de Recherche contre le Cancer" (ARC, project NanoISI), Lab Ex HEPSYS (ANR-10-LAB-28), France. F.C. acknowledges the Australian Research Council for the support under the Australian Laureate Fellowship (120100030, F.C.) and Super Science Fellowship (FS110200025, F.C.) schemes.